Extracellular vesicles
from bone marrow-derived MSCs
Extracellular vesicles (EVs) are produced by telomerized bone marrow mesenchymal stromal cells (BM-MSC/TERT292), and are collected from the EVscale™ perfusion culture harvests.
The EVscale™ platform, created through the collaboration of Evercyte, Phoenestra, and TAmiRNA, delivers a one-stop solution for pharmaceutical and biotech companies needing EVs at a commercial scale.
Leveraging the joint expertise of these partners, EVscale™ offers customizable projects that encompass specific cell sourcing, EV characterization, scalable production, and the correlation of EV biological functions with their RNA and protein components.
General information
Cat#: EV-CHT-063-0292
Presence of typical EV surface marker proteins
EVs derived from BM-MSC/TERT292 cells carry typical surface proteins such as CD81, CD9 and CD63 as demonstrated using CD81+ bead-based flow cytometry. Delta mean fluorescence intensity (DMFI) was calculated by normalizing to MFI of control beads.
Anti-inflammatory activity of extracellular vesicles from
BM-MSC/TERT292
Treatment of mouse macrophage cells (RAW264.7) with lipopolysaccharide (LPS) induces the formation of nitric oxide (NO) indicating an inflammatory reaction. Addition of the positive control Dexamethasone significantly reduces NO secretion.
Similarly, addition of EVs derived from BM-MSC/TERT292 cells significantly reduces NO formation indicating an anti-inflammatory activity of the EVs.
Negative control: buffer; Positive control: dexamethasone.
Anti-fibrotic activity of extracellular vesicles from
BM-MSC/TERT292
Treatment of human fibroblasts (fHDF/TERT166) with Transforming Growth Factor beta (TGF-ß1) induces the expression of alpha smooth muscle actin (α-SMA) indicating myofibroblast differentiation/activation, which is a key event in physiological and pathological tissue repair.
Addition of EVs derived from BM-MSC/TERT292 cells significantly reduces α-SMA induction, indicating an anti-fibrotic activity of the EVs.
Negative control: buffer; Positive control: kinase inhibitor.
FAQs
Upon arrival immediately transfer the product to -80°C.
Store product at -80°C (for up to 6 months) until use.
Thaw the EVs on ice, centrifuge before opening the tube to ensure that the solution is collected at the bottom of the tube. Then, mix carefully by pipetting up and down and aliquot for further use to avoid multiple freeze thaw cycles.
Store the aliquots at -80°C until use.
After thawing, store the EVs at 4°C for a maximum of 1 day.
Product data sheet – certificate of analysis
Data on Markers and Functions
Applications
Selected publications: exosomes for the treatment of COVID-19
Sengupta V, Sengupta S, Lazo A, Woods P, Nolan A, Bremer N. (2020) Exosomes Derived from Bone Marrow Mesenchymal Stem Cells as Treatment for Severe COVID-19. Stem Cells Dev. 2020 Jun 15;29(12):747-754. https://pubmed.ncbi.nlm.nih.gov/32425691/
Selected publications: exosomes with neuro-regenerative properties
Jalilian, E., Massoumi, H., Bigit, B., Amin, S., Katz, E. A., Guaiquil, V. H., Anwar, K. N., Hematti, P., Rosenblatt, M. I., & Djalilian, A. R. (2022). Bone marrow mesenchymal stromal cells in a 3D system produce higher concentration of extracellular vesicles (EVs) with increased complexity and enhanced neuronal growth properties. Stem cell research & therapy, 13(1), 425. https://pubmed.ncbi.nlm.nih.gov/35986305/
Selected publications: exosomes for the treatment of wounds
Saccu, G., Menchise, V., Gai, C., Bertolin, M., Ferrari, S., Giordano, C., Manco, M., Dastrù, W., Tolosano, E., Bussolati, B., Calautti, E., Camussi, G., Altruda, F., & Fagoonee, S. (2022). Bone Marrow Mesenchymal Stromal/Stem Cell-Derived Extracellular Vesicles Promote Corneal Wound Repair by Regulating Inflammation and Angiogenesis. Cells, 11(23), 3892. https://pubmed.ncbi.nlm.nih.gov/36497151/
Casado-Díaz A, Quesada-Gómez JM, Dorado G. (2020) Extracellular Vesicles Derived From Mesenchymal Stem Cells (MSC) in Regenerative Medicine: Applications in Skin Wound Healing. Front Bioeng Biotechnol. 8:146. https://pubmed.ncbi.nlm.nih.gov/32195233/
Customer Reviews
“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”
Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.
“Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform. We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed.”
Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.
Customer Reviews
“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”
Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.
“Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform. We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed.”
Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.
Customer Reviews
“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”
Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.
“Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform. We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed.”
Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.
Customer Reviews
“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”
Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.
“Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform. We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed.”
Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.