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testing anti-fibrotic activity

Chronic inflammation and tissue repair may lead to excessive accumulation of components of the extracellular matrix and subsequently to fibrosis, a pathological wound healing process, changing normal tissue architecture and function. Evercyte has established relevant and standardizable fibroblast cell lines that can be differentiated towards myofibroblasts and therefore represent relevant and standardizable in vitro model systems for fibrosis that will drive the development of new therapeutic approaches.

General information

In vitro model: Evercyte has generated telomerized fibroblast cell lines (dermal skin fibroblasts fHDF/TERT166, lung fibroblasts LF/TERT309) that can be grown without limitations while maintaining a primary-like phenotype.

Assay set-up: treatment of telomerized fibroblasts with transforming growth factor ß (TGFß) induces the expression of alpha smooth muscle actin (alpha-SMA), which is a key event in physiological and pathological tissue repair. Using immunofluorescent staining, alpha-SMA expression upon TGFß treatment alone or in combination with potential anti-fibrotic compounds is analysed.
Timelines:
depending on the number of compounds to be tested, the number of replicates and concentrations requested, it takes between 3 and 5 weeks to deliver results.

Testing anti-fibrotic activity of compounds / EVs

myofibroblast differentiation

Treatment of human dermal fibroblasts (fHDF/TERT166) with transforming growth factor ß (TGF-ß) induces the expression of alpha smooth muscle actin (alpha-SMA / green fluorescence, cell nuclei are counterstained with DAPI), which is a key event in physiological and pathological tissue repair.

Extracellular vesicles from mesenchymal stem cells significantly reduce expression of alpha-SMA after TGF-ß treatment (right figure). These data indicate that EVs from mesenchymal stem cells inhibit myofibroblast differentiation and might exert anti-fibrotic activity.

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Customer Reviews

“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”

 

Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.

"Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform.  We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed."

Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.

all about our
EV cell factories

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fHDF/TERT166

telomerized human skin fibroblasts that can be differentiated towards myofibroblasts

human telomerized lung fibroblasts coming soon!

read more

Customer Reviews

“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”

 

Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.

"Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform.  We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed."

Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.

Related Services

characterization of extracellular vesicles / exosomes to exert anti-fibrotic activity (inhibit myofibroblast differentiation of human fibroblasts from lung or skin)

specific request

fHDF/TERT166

telomerized human skin fibroblasts that can be differentiated towards myofibroblasts

human telomerized lung fibroblasts coming soon!

read more

Related Services

characterization of extracellular vesicles / exosomes to exert anti-fibrotic activity (inhibit myofibroblast differentiation of human fibroblasts from lung or skin)

specific request

Customer Reviews

“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”

 

Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.

"Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform.  We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed."

Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.

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