Screening for substances with senolytic activity

monitoring the cytotoxic effect of exogenous factors on stress-induced premature senescent cells (SIPS)

application: identification of drugs (senolytics) with the potential to block age-related loss of tissue functionality by eliminating accumulating senescent cells

treatment of normal human cells with doxorubicin and induction of stress-induced premature senescence (SIPS)

confirmation of senescent phenotype by analysis of transcriptome (RNAseq)significant overexpression of p21 in SIPS compared to quiescent cells (analysis of cell lysates from 5 different human cell types, 3 donors each)use of senescent cells for testing senolytic activity of drugs