Study of myofibroblast differentiation

monitoring the effect of exogenous factors on myofibroblast differentiation in a model using TGFß as inducer of alpha smooth muscle actin in fibroblasts

application: identification of drugs that influence myofibroblast differentiation during wound healing, fibrosis and fibrocontractive diseases

treatment of telomerized human fibroblasts fHDF/TERT166 with transforming growth factor beta (TGFß) and induction of alpha smooth muscle actin (𝛂-SMA) expression (immunofluorescence staining, cell nuclei are counterstained with DAPI)

reduction of 𝛂-SMA upon treatment with extracellular vesicles from Wharton´s Jelly MSCs