Services

testing wound-healing activity

Cell migration is an important physiological process during embryogenesis, tissue regeneration and wound-healing, but is also associated with pathological situation such as tumor development, metastasis or inflammation.

In order to screen for substances that favor or inhibit cell migration the scratch assay is widely used. Evercyte offers services to test cell migration into a physical gap and test wound-healing activities of certain compounds.

General information

In vitro model: Evercyte has generated telomerized human fibroblast cells (e.g. fHDF/TERT166 from foreskin, HDF/TERT164 from adult skin, LF/TERT309 from lung) that can be grown without limitation while maintaining a primary-like phenotype.

Assay set-up: fibroblasts are seeded into chamber slides and a physical gap within the monolayer is created followed by monitoring the process of cell migration into the gap. Gap closure is quantified as average free area with or without mitomycin C treatment for discrimination between cell migration and proliferation.
Timelines:
depending on the number of samples to be tested, the number of replicates and concentrations it takes 2-6 weeks to deliver results.

Testing wound-healing activity of extracellular vesicles

fibroblast growth promoting activity / scratch assay

Telomerized human fibroblasts (fHDF/TERT166) are seeded into chamber slides and a physical gap within the monolayer is created followed by monitoring the process of cell migration into the gap. Significantly less free area between the cells is detected upon addition of extracellular vesicles from mesenchymal stem cells (WJ-MSC/TERT273).

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testing the potency of extracellular vesicles / exosomes

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fHDF/TERT166

telomerized human foreskin fibroblasts

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MSCs for production of clinical grade extracellular vesicles

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Customer Reviews

“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”

 

Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.

"Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform.  We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed."

Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.

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EV cell factories

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fHDF/TERT166

telomerized human skin fibroblasts are a relevant and standardizable model system to study wound healing activities in vitro

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Related Services

characterization of extracellular vesicles / exosomes to exert fibroblast growth promoting / wound healing activity using scratch assay

specific request

Customer Reviews

“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”

 

Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.

"Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform.  We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed."

Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.

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