Services
characterization of exosomes
Extracellular vesicles (EVs) play an essential role in cellular communication by transporting proteins, lipids as well as nucleic acids. Evercyte offers the production of extracellular vesicles from customer-derived cells as well as from telomerized Evercyte cells as well as in depth characterization for presence of typical marker proteins, morphological characterizstics and in vitro potency.
General information
Determination of particle number and size: Evercyte uses nanoparticle tracking analysis (NTA, particle metrix) for determination of particle number and size
Morphology of EVs: Cryo-electron microscopy is used to characterize EVs for the typical lipid double layer membrane
Flow cytometry for determination of presence of EV marker proteins
Extracellular vesicles from telomerized mesenchymal stem cells and the corresponding non-transfected normal cells are characterized for presence of typical marker proteins such as CD63 and CD81. These data were generated by incubation of EVs with exosome-human CD81 beads (invitrogen) followed by staining with specific antibodies.
Morphological characterization of extracellular vesicles
cryo-electron microscopy
The morphology of extracellular vesicles from telomerized mesenchymal stem cells was analysed using cryo-electron microscopy. Thereby generated data show that EVs from these cells are characterized by the typical lipid double layer membrane.
Customer Reviews
“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”
Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.
"Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform. We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed."
Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.
Customer Reviews
“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”
Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.
"Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform. We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed."
Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.
