Determination of particle number and size: Evercyte uses nanoparticle tracking analysis (NTA, particle metrix) for determination of particle number and size
Presence of marker proteins : Evercyte uses western blotting as well as multiplex flow cytometry for testing the presence of EV specific marker proteins (e.g. CD9, CD63, CD81)
Morphology of EVs: Cryo-electron microscopy is used to characterize EVs for the typical lipid double layer membrane
Flow cytometry for determination of presence of EV marker proteins
Extracellular vesicles from telomerized mesenchymal stem cells and the corresponding non-transfected normal cells are characterized for presence of typical marker proteins such as CD63 and CD81. These data were generated by incubation of EVs with exosome-human CD81 beads (invitrogen) followed by staining with specific antibodies.
Morphological characterization of extracellular vesicles
The morphology of extracellular vesicles from telomerized mesenchymal stem cells was analysed using cryo-electron microscopy. Thereby generated data show that EVs from these cells are characterized by the typical lipid double layer membrane.
Leaflets - extracellular vesicles