Knock-out of CD46 in RPTEC/TERT1 cells

Application: gene editing of human telomerized cells with CRISPR/Cas9

knock-out of CD46 in RPTEC/TERT1 cells using CRISPR / Cas9, selection of homozygous knock-out clones (-/-), confirmation of CD46 knock-out in clone 1E3 (lower right)

characterization of RPTEC/TERT1 CD46 knock-out clone confirmation of maintenance of typical markers of renal proximal tubular epithelial cells (cobblestone morphology, dome formation, expression of ZO-1 and C-cadherin)