Overexpression of green fluorescent protein in endothelial cells

Application: establishment of reporter cell lines from relevant and standardizable telomerized human cells

transfection of telomerized human umbilical vein endothelial cells HUVEC/TERT2 with a plasmid carrying green fluorescence protein and selection of transfectants

characterization of stable GFP expressing cells for maintenance of cell type specific functions
no loss in ability to form tubule-like structures on Matrigel matrix