Can the medium be stored for more than 1 month?
The medium contains temperature sensitive ingredients and therefore it is not recommended to use the medium for a prolonged period of time. Moreover, we recommend our customers to
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06, 2021
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06, 2021
What is the difference between PODO/TERT256 and PODO/SVTERT152 cells?
PODO/TERT256 cells, isolated from kidney tissue biopsies have been immortalized by reactivation of telomerase alone, whereas urine-derived PODO/SVTERT152 cells overexpress the catalytic subunit of human telomerase together with
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06, 2021
Is it necessary to cultivate the cells in the presence of G418?
G418 was used to select for stable positively transfected cells. Even if we have not systematically tested the stability of PODO/TERT256 cells when cultivated without G418, we assume
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06, 2021
Can the medium be stored for more than 1 month?
The medium contains temperature sensitive ingredients and therefore it is not recommended to use the medium for a prolonged period of time. Moreover, we recommend our customers to
21
06, 2021
Can you provide a protocol for transfection of the cells?
The cells can be transfected using electroporation. Using Neon Transfection System (Thermo Fisher, settings 1100/30/2) we can reach about 30 % transfection efficiency.
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06, 2021
What is the difference between HUVEC/TERT2 and HUVEC/TERT66 cells?
HUVEC/TERT2 cells are grown in a medium containing animal-derived products (e.g. bovine brain extract and fetal bovine serum), which show high lot-to-lot variabilities. On the contrary, HUVEC/TERT66 cells
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06, 2021
What is the difference between fHDF/TERT166 and HDF/TERT164 cells?
fHDF/TER166 cells are derived from foreskin, whereas HDF/TERT164 cells are from adult skin (Cutis Laxa). Both cell lines show typical markers and characteristics of fibroblastoid cells and a
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06, 2021
Can we use the extracellular vesicles for clinical application?
No, the EVs cannot be used in humans. However, Evercyte EV production cell lines qualify for production of clinical grade material. Please contact us for more detailed information
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06, 2021
Can I use the the exosomes/extracelluar vesicles for sensitive cell cultures?
Yes. Our exosomes/extracelluar vesicles do not carry contaminations (bacteria incl. mycoplasma, fungi) or endotoxins and have been tested for their activity on primary like cells.
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06, 2021
Can the medium be stored for more than 1 month?
The medium contains temperature sensitive ingredients and therefore it is not recommended to use the medium for a prolonged period of time. Moreover, we recommend our customers to