Do I have to add antibiotics to the medium?
We advise to use media without antibiotics for routine cell culture. Please also see "Guidance on Good Cell Culture Practice" by Coecke et al. (2005) https://pubmed.ncbi.nlm.nih.gov/16180980.
By Evercyte|2021-10-08T09:41:21+00:00October 8th, 2021|antibiotics|
We advise to use media without antibiotics for routine cell culture. Please also see "Guidance on Good Cell Culture Practice" by Coecke et al. (2005) https://pubmed.ncbi.nlm.nih.gov/16180980.
By admin|2024-07-02T11:20:24+00:00June 21st, 2021|EVproductioncells|
Yes, a cell bank was established that can be transferred to a GMP facility for establishment of a production master cell bank. The cell line was established under
By admin|2021-06-21T09:45:25+00:00June 21st, 2021|RPTEC/TERT1|
The cells can be transfected using Lipofectamine 2000 as described in Wieser M, et al. (2019) CD46 knock-out using CRISPR/Cas9 editing of hTERT immortalized human cells modulates complement
By admin|2021-06-21T09:43:49+00:00June 21st, 2021|RPTEC/TERT1|
NGS data of undifferentiated cells have been generated by the Human Protein Atlas ® (https://www.proteinatlas.org/search/RPTEC%2FTERT1)
By admin|2021-06-21T09:36:12+00:00June 21st, 2021|PodoUp|
The medium contains temperature sensitive ingredients and therefore it is not recommended to use the medium for a prolonged period of time. Moreover, we recommend our customers to
By admin|2021-06-21T09:29:19+00:00June 21st, 2021|PODO/SVTERT256|
PODO/TERT256 cells, isolated from kidney tissue biopsies have been immortalized by reactivation of telomerase alone, whereas urine-derived PODO/SVTERT152 cells overexpress the catalytic subunit of human telomerase together with
By admin|2021-06-21T09:28:28+00:00June 21st, 2021|PODO/SVTERT256|
G418 was used to select for stable positively transfected cells. Even if we have not systematically tested the stability of PODO/TERT256 cells when cultivated without G418, we assume
By admin|2021-06-21T08:41:57+00:00June 21st, 2021|MyoUp|
The medium contains temperature sensitive ingredients and therefore it is not recommended to use the medium for a prolonged period of time. Moreover, we recommend our customers to
By admin|2021-06-21T08:37:56+00:00June 21st, 2021|LHCN-M2|
The cells can be transfected using electroporation. Using Neon Transfection System (Thermo Fisher, settings 1100/30/2) we can reach about 30 % transfection efficiency.
By admin|2021-10-08T06:32:56+00:00June 21st, 2021|huvec/tert2, huvec/tert66faq|
HUVEC/TERT2 cells are grown in a medium containing animal-derived products (e.g. bovine brain extract and fetal bovine serum), which show high lot-to-lot variabilities. On the contrary, HUVEC/TERT66 cells