By Evercyte|2021-12-15T09:34:44+00:00December 15th, 2021|cell line development, immortalization|
Ectopic expression of the catalytic subunit of human telomerase hTERT leads to an extension of the cellular life span while maintaining cell type specific markers and functions. Therefore,
By Evercyte|2021-12-15T09:05:13+00:00December 15th, 2021|cell line development, primary cells|
The number of population doublings that can be performed strongly depends on several parameters such as the cell type, the culture conditions as well as the number of
By Evercyte|2021-12-01T14:28:41+00:00December 1st, 2021|ASC/TERT, EV production cell line|
Both cell lines originate from adipose tissue from the same donor. While ASC/TERT300 is grown in xeno-free Mesencult medium, ASC/TERT300-B cell lines is grown in a medium containing
By Evercyte|2021-10-13T17:46:35+00:00October 13th, 2021|ASC/TERT|
Both cell lines originate from adipose tissue. While ASC/TERT300 was manufactured under conditions that will allow later use for production of GMP grade extracellular vesicles, ASC/TERT1 is used
By Evercyte|2021-10-08T09:41:21+00:00October 8th, 2021|antibiotics|
We advise to use media without antibiotics for routine cell culture. Please also see "Guidance on Good Cell Culture Practice" by Coecke et al. (2005) https://pubmed.ncbi.nlm.nih.gov/16180980.
By admin|2024-08-23T08:26:15+00:00June 21st, 2021|EVproductioncells|
Yes, a cell bank was established that can be transferred to a GMP facility for establishment of a production master cell bank. The cell line was established under
By admin|2021-06-21T09:45:25+00:00June 21st, 2021|RPTEC/TERT1|
The cells can be transfected using Lipofectamine 2000 as described in Wieser M, et al. (2019) CD46 knock-out using CRISPR/Cas9 editing of hTERT immortalized human cells modulates complement
By admin|2021-06-21T09:43:49+00:00June 21st, 2021|RPTEC/TERT1|
NGS data of undifferentiated cells have been generated by the Human Protein Atlas ® (https://www.proteinatlas.org/search/RPTEC%2FTERT1)
By admin|2021-06-21T09:36:12+00:00June 21st, 2021|PodoUp|
The medium contains temperature sensitive ingredients and therefore it is not recommended to use the medium for a prolonged period of time. Moreover, we recommend our customers to
By admin|2021-06-21T09:29:19+00:00June 21st, 2021|PODO/SVTERT256|
PODO/TERT256 cells, isolated from kidney tissue biopsies have been immortalized by reactivation of telomerase alone, whereas urine-derived PODO/SVTERT152 cells overexpress the catalytic subunit of human telomerase together with
