The cells can be transfected using Lipofectamine 2000 as described in Wieser M, et al. (2019) CD46 knock-out using CRISPR/Cas9 editing of hTERT immortalized human cells modulates complement activation. PLoS One. 2019; 14(4): e0214514. www.ncbi.nlm.nih.gov/pmc/articles/PMC6453361