ColoUp2021-08-01T20:24:45+00:00

Colon

ColoUp

ColoUp medium support the growth of Evercyte ́s human colon epithelial cell line HCEC-1CT.

General information

Cat#: MHT-039 / MHT-039-B + MHT-039-S

Quantity: 500 ml
Format: ready-to-use medium (MHT-039) or kit (basal medium / MHT-039-B + supplements / MHT-039-S)
Storage temperature:
ready-to-use medium / 4°C, basal medium / 4°C, supplements / -20°C
Shelf life:
ready-to-use medium / 1 month, basal medium / 6 months, 4°C, supplements / 6 months
Application:
growth of human colon epithelial cells HCEC-1CT (Evercyte, Cat# CHT-039-0229)

Medium composition

DMEM (Gibco, Cat # 10566016) / M199 (Gibco, Cat# 31150022) (4+1),
2 % Cosmic Calf Serum (Hyclone, Cat# SH30087, ready-to-use)
20 ng/ml hEGF (Sigma-Aldrich, Cat# E9644)
10 µg/ml Insulin (Sigma-Aldrich, Cat# I9278, ready-to-use)
2 µg/ml Apo-Transferrin (Sigma-Aldrich, Cat# T2036)
5 nM Sodium-Selenite (Sigma-Aldrich, Cat# S5261)
1 µg/ml Hydrocortisone (Sigma-Aldrich, Cat# H0396)

FAQs

Can the medium be stored for more than 1 month?2021-06-21T07:29:54+00:00

The medium contains temperature sensitive ingredients and therefore it is not recommended to use the medium for a prolonged period of time. Moreover, we recommend our customers to aliquot the medium and store aliquots at 4°C until use. Before use, temper the aliquots at room temperature (not at 37°C).

Preparation of ColoUp medium

The ColoUp medium for cultivation of HCEC-1CT cells can either be ordered from Evercyte as ready-to-use medium (Cat#MHT-039) or as basal medium (MHT-039-B) + Supplements (MHT-039-S) or can be prepared by mixing the following components:

take one bottle (500 ml) of DMEM (Gibco, Cat # 10566016) and discard 100 ml
add 100 ml of M199 (Gibco, Cat# 31150022) and mix properly
discard 11.6 ml DMEM/M199 mixture
add 10 ml Cosmic Calf Serum (ready-to-use, Hyclone, Cat# SH30087)
add 500 µl of hEGF stock (20 µg/ml, prepared in cell culture grade water, Sigma-Aldrich, Cat# E9644)
add 500 µl of Insulin (10 mg/ml, ready-to-use, Sigma-Aldrich, Cat# I9278)
add 100 µl of Apo-Transferrin stock (10 mg/ml, prepared in M199, Sigma-Aldrich, Cat# T2036)
add 25 µl of Sodium-Selenite stock (100 µM, prepared in cell culture grade water, Sigma-Aldrich, Cat# S5261)
add 500 µl Hydrocortisone stock (1 mg/ml, prepared in cell culture grade water, Sigma-Aldrich, Cat# H0396)
mix properly and store at 4°C for up to 1 month
temper the medium to room temperature (not 37°C) before use
Protocol preparation of ColoUp medium

Storage conditions

Store the basal medium at 4°C for up to 6 months and the supplements -20°C for up to 6 months.

Store the ready-to-use medium in aliquots at 4°C for up to 1 month..
Temper the medium to room temperature (not 37°C) before use.
Avoid repeated heating to 37°C.
Protocol passaging of HCEC-1CT

Protocols

Preparation of cell culture medium ColoUp
Protocol passaging of HCEC-1CT

Safety documents (coming soon)

Cell culture media – material safety data sheet

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ColoUp
Ready-to-use – € 135.90,–

Kit components – € 183.60,–

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Related Products

human colon epithelial cells

HCEC-1CT

read more

Related Services

send us specific request

Customer Reviews

“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”

 

Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.

“Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform.  We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed.”

Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.

about
our cells

telomerized cells for the establishment of relevant and standardizable in vitro tests

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news

Lecture on 3Rs

(replacement – reduction -refinement of animal experiments)

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Related Products

HCEC-1CT

Related Services

specific request

Customer Reviews

“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”

 

Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.

“Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform.  We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed.”

Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.

Print Friendly, PDF & Email

ColoUp
Cat#: MHT-039 – € 135.90,–

Cat#: MHT-039-B – € 89.60,–

Cat#: MHT-039-S – € 94.00,–

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