Colon
HCEC-1CT
Evercyte ́s human colon epithelial cell line HCEC-1CT can be grown without limitations while maintaining expression of cell type specific markers and functions. Therefore, these cells are useful for setting up in vitro bioassays to study drug / nutrient absorption and to assess drug-induced toxicity on the large intestine barrier. Additionally, the cells are valuable for modelling cancer progression in vitro.
General information
Cat#: CkHT-039-0229
Morphology and expression of marker proteins
HCEC-1CT cell line shows the typical epithelial, cobblestone appearance and expression of characteristic, colonic epithelial markers such as A33, Mucin-1 as well as moderate expression of Villin in 2D differentiated cultures. Cell nuclei are counterstained with DAPI.
Cell-cell contacts in 3D spheroid culture

When grown as 3D spheroids, HCEC-1CT cells form multicellular organoid structures that stain positive for ZO1 demonstrating formation of cell-cell contacts. Cell nuclei are counterstained with DAPI.
FAQs
In vitro propagation
ColoUp (Evercyte, Cat# MHT-039)
DMEM (Gibco, Cat# 10566016)/M199 (Gibco, Cat# 31150022) (4+1)
2 % Cosmic Calf Serum (Hyclone, Cat# SH30087)
20 ng/ml EGF (Sigma Aldrich, Cat# E9644)
10 µg/ml Insulin (Sigma Aldrich, Cat# I9278)
2 µg/ml Apo-Transferrin (Sigma Aldrich, Cat# T2036)
5 nM Sodium-Selenit (Sigma-Aldrich, Cat# S5261)
Additional material & reagents
Phosphate buffered saline (PBS) (Sigma, Cat# D8537)
Trypsin inhibitor (Gibco, Cat# R007100)
0,05 % Trypsin-EDTA (Gibco, Cat#25300-054)
Passaging of cells
For detachment of the cells remove and discard the culture medium and wash the cells once with PBS (about 160 µl/cm²). Remove PBS completely.
Cryopreservation
Freezing medium
ColoUp growth medium (Evercyte, Cat# MHT-039)
10% DMSO (Sigma Aldrich, Cat# D2650)
10% Cosmic Calf Serum (Hyclone, Cat# SH30087)
Additional material & reagents
0,05 % Trypsin-EDTA (Gibco, Cat# 25300-054)
Trypsin inhibitor (Gibco, Cat# R007100)
Freezing of cells
Detach the cells from the culture vessel by using Trypsin-EDTA and Trypsin-Inhibitor (Protocol passaging of HCEC-1CT).
Thawing of cells
Original Evercyte cells are to be thawed in a T25 roux flask
Product data sheet – certificate of analysis
Protocols
Data on markers and functions
Safety documents
Coming soon.
Selected publications
Scavo MP, Rizzi F, Depalo N, Fanizza E, Ingrosso C, Curri ML, Giannelli G. A Possible Role of FZD10 Delivering Exosomes Derived from Colon Cancers Cell Lines in Inducing Activation of Epithelial-Mesenchymal Transition in Normal Colon Epithelial Cell Line. Int J Mol Sci. 2020 Sep 3;21(18):6705. https://pubmed.ncbi.nlm.nih.gov/32933173/
Licence Conditions
The business concept of Evercyte is to out-license telomerized cells to our customers. The license conditions depend on whether the contract partner is a for profit or a nonprofit organization and the intended use of the cells.
Nonprofit organizations
On time payment for unlimited use: EUR 1600
Profit organizations
Pharmaceutical – chemical – cosmetic industries
Contract research organizations (CRO)
Initial license fee for 3 months: EUR 2500Annual license fee R&D: royalty based
Customer Reviews
“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”
Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.
“Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform. We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed.”
Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.

Determination of IC50 values of compounds using relevant human telomerized cells
Customer Reviews
“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”
Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.
“Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform. We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed.”
Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.