3D spheroid assay for screening of modulation of angiogenesis

monitoring the effect of extracellular vesicles on sprout formation from endothelial spheroid

application: identification of extracellular vesicles that modify angiogenesis / treatment option for vascular malfunction-associated diseases

cultivation of telomerized human umbilical vein endothelial cells HUVEC/TERT2 as spheroids in hanging drops; embedding of spheroids into semi-sold matrix

treatment of spheroids with vascular endothelial growth factor (VEGF), extracellular vesicles from endothelial cells or Wharton´s Jelly derived MSCs, induction of sprout formation by VEGF and EVs from WJ-MSC/TERT273 cells