In vitro model systems offered: Evercyte´s cytotoxic activity assays are based on human telomerized cells from different tissues and organs (e.g. skin, cornea, lung, vascular system, kidney, placenta). Using these relevant and standardizable cells Evercyte has established cell-based assays to test cellular viability, cell proliferation and cell death mechanisms (e.g. alamarBlue assay, MTT assay, LDH assay, FDA assay, ATP assay, Annexin-V / PI apoptosis assay) What we need:
information on compounds (e.g. diluent, concentrations to be tested, number of replicates, IC50 measurements required) Timelines:
depending on the bioassay, the number of samples to be tested, the number of replicates and concentrations, it takes 3-6 weeks to deliver results
Testing cyclosporin A induced cytotoxicity on endothelial cells
testing metabolic activity of treated cells / MTT assay
Telomerized human umbilical vein endothelial cells HUVEC/TERT2 are treated with different concentrations of cyclosporin A and cellular viability is measured using MTT assay. IC50 value is determined after incubation with cyclosporin A for 72 hours.