Services

cytotoxicity testing

The use of standardized and relevant in vitro model systems is of ever increasing importance for testing drug-induced toxicity as well as the potential of drugs to boost cellular proliferation.

Using relevant and standardizable human telomerized cell lines, Evercyte offers services for testing drugs for a potential cytotoxic activity, whereby several methods are applied to determine the cell death mechanism. This allows to screen and rank compounds for further testings.

General information

In vitro model systems offered: Evercyte´s cytotoxic activity assays are based on human telomerized cells from different tissues and organs (e.g. skin, cornea, lung, vascular system, kidney, placenta). Using these relevant and standardizable cells Evercyte has established cell-based assays to test cellular viability, cell proliferation and cell death mechanisms (e.g. alamarBlue assay, MTT assay, LDH assay, FDA assay, ATP assay, Annexin-V / PI apoptosis assay)

What we need:  information on compounds (e.g. diluent, concentrations to be tested, number of replicates, IC50 measurements required)
Timelines:
depending on the bioassay, the number of samples to be tested, the number of replicates and concentrations, it takes 3-6 weeks to deliver results

Testing cyclosporin A induced cytotoxicity on endothelial cells

testing metabolic activity of treated cells / MTT assay  

Telomerized human umbilical vein endothelial cells HUVEC/TERT2  are treated with different concentrations of cyclosporin A and cellular viability is measured using MTT assay. IC50 value is determined after incubation with cyclosporin A for 72 hours.

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Customer Reviews

“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”

 

Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.

“Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform.  We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed.”

Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.

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Customer Reviews

“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”

 

Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.

“Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform.  We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed.”

Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.

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