MyoUp2021-08-01T20:49:42+00:00

Muscle

MyoUp

MyoUp medium support the growth of Evercyte ́s human mycoblast cell line LHCN-M2.

General information

Cat#: MHT-040

Quantity: 500 ml
Format: ready-to-use medium
Storage temperature:
4°C
Shelf life:
1 month
Application:
growth of human muscle cells LHCN-M2 (Evercyte, Cat# CkHT-040-231-2)

Medium composition

DMEM (Gibco, Cat # 10566016) / M199 (Gibco, Cat# 31150022) (4+1)

15 % FBS (Sigma-Aldrich, Cat# F7524)

20 mM Hepes (Sigma-Aldrich, Cat# H0887)

0.03 µg/ml Zinc sulfate (Sigma-Aldrich, Cat# Z0251)

1.4 µg/ml Vitamin B12 (Sigma-Aldrich, Cat# V2876)

0.055 µg/ml Dexamethasone (Sigma-Aldrich, Cat# D4902)

2.5 ng/ml HGF (Merck Millipore, Cat# GF116)

10 ng/ml bFGF (Peprotech, Cat# 100-18B)

FAQs

Can the cells be used for production of clinical grade extracellular vesicles?2021-06-21T09:54:55+00:00

Yes, a cell bank was established that can be transferred to a GMP facility for establishment of a production master cell bank. The cell line was established under xeno-free conditions with full documentation of any manipulation step. Please contact us for more detailed information on our production cell line.

Preparation of MyoUp medium

The MyoUp medium for cultivation of LHCN-M2 cells can either be ordered from Evercyte as ready-to-use medium (Cat#MHT-040) or can be prepared by mixing the following components:

take one bottle (500 ml) of DMEM (Gibco, Cat # 10566016) and discard 100 ml

add 100 ml of M199 (Gibco, Cat# 31150022) and mix properly

discard 90 ml DMEM/M199 mixture

add 75 ml FBS (ready-to-use, Sigma-Aldrich, Cat# F7524)

add 10 ml Hepes (1M stock, ready-to-use, Sigma-Aldrich, Cat# H0887)

add 50 µl Zink Sulfate stock (30 mg/ml, prepared in cell culture grade water, Sigma-Aldrich, Cat# Z0251)

add 50 µl Vitamin B12 stock (14 mg/ml, prepared in cell culture grade water, Sigma-Aldrich, Cat# V2876)

add 70 µl Dexamethasone stock (1 mM, prepared in absolute ethanol/DMEM, Sigma-Aldrich, Cat# D4902)

add 25 µl HGF stock (50 µg/ml, prepared in cell culture grade water, Merck Millipore, Cat# GF116)

add 50 µl bFGF stock solution (100 µg/ml, prepared in PBS/Tris/BSA buffer, Peprotech, Cat# 100-18B)

mix properly and store at 4°C for up to 1 month

Protocol preparation of MyoUp medium

Storage conditions

Store the basal medium at 4°C for up to 6 months and the supplements -20°C for up to 6 months.

Store the ready-to-use medium in aliquots at 4°C for up to 1 month..
Temper the medium to room temperature (not 37°C) before use.
Avoid repeated heating to 37°C.
Protocol passaging of LHCN-M2 cells

Protocols

Preparation of cell culture medium MyoUp
Protocol passaging of LHCN-M2

Safety documents (coming soon)

Cell culture media – material safety data sheet

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MyoUp
Ready-to-use – € 195.30,–

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Customer Reviews

“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”

 

Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.

“Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform.  We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed.”

Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.

about
our cells

telomerized cells in biotechnology and biomedicine

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Good cell culture practice / GCCP

get ready to standardize your cell culture work

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Related Products

MyoUp MHT-040

Related Services

specific request

Customer Reviews

“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”

 

Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.

“Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform.  We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed.”

Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.

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MyoUp
Cat#: MHT-040 – € 195.30,–

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