Muscle
MyoUp2
MyoUp medium support the growth of Evercyte’s human myoblast cell line LHCN-M2.
MyoUp2 medium contains all components of MyoUp but FBS. Before use, MyoUp2 medium has to be supplemented with 15% FBS to support growth of LHCN-M2 cells.
General information
Cat#: MHT-040-02
Medium composition
DMEM (Gibco, Cat # 61965-026) / M199 (Gibco, Cat# 31150022) (4+1)
20 mM Hepes (Sigma-Aldrich, Cat# H0887)
3 µg/ml Zinc sulfate (Sigma-Aldrich, Cat# Z0251)
1.4 µg/ml Vitamin B12 (Sigma-Aldrich, Cat# V2876)
0.055 µg/ml Dexamethasone (Sigma-Aldrich, Cat# D4902)
2.5 ng/ml HGF (Merck Millipore, Cat# GF116)
10 ng/ml bFGF (Peprotech, Cat# 100-18B)
Before use MyoUp2 medium must be supplemented with 15 % FBS (e.g.PAN Biotech, Cat# P30-3031 or Sigma, Cat# F7524 )
FAQs
Preparation of MyoUp medium
The MyoUp medium for cultivation of LHCN-M2 cells contains the herein described components and is prepared as described in the following:
take one bottle (500 ml) of DMEM (Gibco, Cat # 61965-026) and discard 100 ml
add 10 ml Hepes (1M stock, ready-to-use, Sigma-Aldrich, Cat# H0887)
add 50 µl Zink Sulfate stock (30 mg/ml, prepared in cell culture grade water, Sigma-Aldrich, Cat# Z0251)
add 50 µl Vitamin B12 stock (14 mg/ml, prepared in cell culture grade water, Sigma-Aldrich, Cat# V2876)
add 70 µl Dexamethasone stock (1 mM, prepared in absolute ethanol/DMEM, Sigma-Aldrich, Cat# D4902)
add 25 µl HGF stock (50 µg/ml, prepared in cell culture grade water, Merck Millipore, Cat# GF116)
add 50 µl bFGF stock solution (100 µg/ml, prepared in PBS/Tris/BSA buffer, Peprotech, Cat# 100-18B)
mix properly and store at 4°C for up to 1 month
Storage conditions
Store the ready-to-use medium in aliquots at 4°C for up to 1 month.
Customer Reviews
“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”
Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.
“Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform. We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed.”
Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.
Customer Reviews
“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”
Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.
“Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform. We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed.”
Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.