Tissue, cell type: Evercyte offers the isolation of specific cell types from various tissues and body fluids Ethical issues:
cells are isolated with ethical approval from the responsible IRB and prior given written informed consentIsolation method:
mechanical and/or enzymatic dissagregation of tissue specific cell-cell and cell-matrix contactsCell enrichment:
use of specific cell culture media and coatings, cell sorting using FACS or MACS, cloningCharacterization:
expression of cell type specific markers and function, authentication, freedom from contaminations
Recent example – isolation of amniotic membrane epithelial cells
Amniotic membrane is sepatated from whole placental tissue followed enzymatic tissue digestion. After 7-10 days cultivation in serum-free cell culture medium, small colonies appear showing the typical epithelial morphology. These cells are enriched and stained for presence of typical marker proteins such as SSEA-4 (red fluorescence, DNA is counterstained with DAPI).
Recent example – isolation of chorionic plate MSCs
Chorionic plate region is prepared from whole placental tissue and cut into small pieces. The resulting fragments are seeded into culture plates for outgrowth of mesenchymal stem cells. These cells are enriched in vitro and show the typical spindle-shaped mesenchymal morphology.