Services
primary cell culture
With more that 25 years of experience in human tissue disaggregation and selection of specific cell types, Evercyte offers services to establish human primary cell cultures from various tissues and organs. The studies are performed with ethical approval from the local ethical committee and prior given written informed consent by the donor.
General information
Tissue, cell type: Evercyte offers the isolation of specific cell types from various tissues and body fluids
Recent example – isolation of amniotic membrane epithelial cells
Amniotic membrane is sepatated from whole placental tissue followed enzymatic tissue digestion. After 7-10 days cultivation in serum-free cell culture medium, small colonies appear showing the typical epithelial morphology. These cells are enriched and stained for presence of typical marker proteins such as SSEA-4 (red fluorescence, DNA is counterstained with DAPI).
Recent example – isolation of chorionic plate MSCs
Chorionic plate region is prepared from whole placental tissue and cut into small pieces. The resulting fragments are seeded into culture plates for outgrowth of mesenchymal stem cells. These cells are enriched in vitro and show the typical spindle-shaped mesenchymal morphology.
FAQs
Customer Reviews
“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”
Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.
"Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform. We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed."
Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.
Customer Reviews
“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”
Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.
"Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform. We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed."
Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.