Kidney
ProxUp3
ProxUp2 medium supports the growth of Evercyte’s human renal proximal tubular epithelial cell line RPTEC/TERT1.
ProxUp3 medium contains all components of ProxUp2 medium but FBS. ProxUp3 medium must be supplemented with 0.5% FBS before use.
General information
Cat#: MHT-003-03 / MHT-003-2-B + MHT-003-S
ProxUp3 medium composition
DMEM/F12 (1:1) (PAN-Biotech, Cat# P04-41154)
10 mM HEPES-buffer (Sigma-Aldrich, Cat# H0887, ready-to-use)
10 ng/ml hEGF (Sigma-Aldrich, Cat# E9644)
5 pM 3,3′,5-Triiodo-L-thyronine sodium salt (T3, Sigma-Aldrich, Cat# T6397)
3.5 µg/ml L-Ascorbic Acid (Sigma-Aldrich, Cat# A4544)
5 µg/ml Transferrin Holo (Merck Millipore, Cat# 616424)
25 ng/ml Prostaglandine E1 (Sigma-Aldrich, Cat# P8908)
25 ng/ml Hydrocortisone (Sigma-Aldrich, Cat# H0396)
8.65 ng/ml Sodium-Selenite (Sigma-Aldrich, Cat# S5261)
5 µg/ml Insulin (Sigma-Aldrich, Cat# I9278, ready-to-use)
100 µg/ml G418 (InvivoGen, Cat# ant-gn-5, ready-to-use)
Before use ProxUp3 medium must be supplemented with 0.5% FBS (e.g. PAN Biotech, Cat# P30-3031 or Sigma, Cat# F7524)
FAQs
Preparation of ProxUp2 medium
The ProxUp2 medium for cultivation of RPTEC/TERT1 cells contains the herein described components and is prepared as described in the following:
take one bottle (500 ml) of DMEM/F12 (1:1) (PAN-Biotech, Cat# P04-41154) |
add 2.5ml of FBS (e.g. PAN Biotech, Cat# P30-3031,)
Storage conditions
Store the basal medium at 4°C for up to 6 months and the supplements -20°C for up to 6 months.
Customer Reviews
“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”
Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.
“Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform. We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed.”
Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.
induction of senescence in human normal cells for testing senolytic activity of novel drugs
Customer Reviews
“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”
Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.
“Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform. We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed.”
Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.