ProxUp2021-08-02T07:16:45+00:00

Kidney

ProxUp

ProxUp medium support the growth of Evercyte ́s human renal proximal tubular epithelial cells RPTEC/TERT1.

General information

Cat#: MHT-003 / MHT-003-B + MHT-003-S

Quantity: 500 ml
Format: ready-to-use medium (MHT-003) or basal medium (MHT-003-B) + supplements (MHT-003-S)
Storage temperature:
ready-to-use medium / 4°C, basal medium / 4°C, supplements / -20°C
Shelf life:
ready-to-use medium / 1 month, basal medium / 6 months, 4°C, supplements / 6 months
Application:
growth of human renal proximal tubular epithelial cells RPTEC/TERT1 (CHT-003-0002)

Medium composition

DMEM/F12 (1:1) (PAN-Biotech, Cat# P04-41154)

10 mM HEPES-buffer (Sigma-Aldrich, Cat# H0887, ready-to-use)

10 ng/ml hEGF (Sigma-Aldrich, Cat# E9644)

5 pM 3,3′,5-Triiodo-L-thyronine sodium salt (T3, Sigma-Aldrich, Cat# T6397)

3.5 µg/ml L-Ascorbic Acid (Sigma-Aldrich, Cat# A4544)

5 µg/ml Transferrin Holo (Merck Millipore, Cat# 616424)

25 ng/ml Prostaglandine E1 (Sigma-Aldrich, Cat# P8908)

25 ng/ml Hydrocortisone (Sigma-Aldrich, Cat# H0396)

8.65 ng/ml Sodium-Selenite (Sigma-Aldrich, Cat# S5261)

5 µg/ml Insulin (Sigma-Aldrich, Cat# I9278, ready-to-use)

100 µg/ml G418 (InvivoGen, Cat# ant-gn-5, ready-to-use)

FAQs

Can the medium be stored for more than 1 month?2021-06-21T08:41:57+00:00

The medium contains temperature sensitive ingredients and therefore it is not recommended to use the medium for a prolonged period of time. Moreover, we recommend our customers to aliquot the medium and store aliquots at 4°C until use. Before use, temper the aliquots at room temperature (not at 37°C).

Preparation of ColoUp medium

The ProxUp medium for cultivation of RPTEC/TERT1 cells can either be ordered from Evercyte as ready-to-use medium (Cat# MHT-003) or as basal medium (MHT-003-B) + Supplements (MHT-003-S) or can be prepared by mixing the following components:

take one bottle (500 ml) of DMEM/F12 (1:1) (PAN-Biotech, Cat# P04-41154
add 5 ml of HEPES (1M, ready-to-use, Sigma-Aldrich, Cat# H0887)

add 250 µl of hEGF stock (20 µg/ml, prepared in cell culture grade water, Sigma-Aldrich, Cat# E9644

add 250 µl of T3 stock (10 nM, prepared in NaOH/PBS, Sigma-Aldrich, Cat# T6397)

add 250 µl of Ascorbic acid stock (7 mg/ml, prepared in cell culture grade water, Sigma-Aldrich, Cat# A4544)

add 250 µl of Transferrin Holo stock (10 mg/ml, prepared in cell culture grade water, Merck Millipore, Cat# 616424)
add 250 µl of Prostaglandine E1 stock (50 µg/ml, prepared in basal medium, Sigma-Aldrich, Cat# P8908)

add 250 µl of Hydrocortisone Stock (50 µg/ml, prepared in cell culture grade water, Sigma-Aldrich, Cat# H0396)

add 250 µl of Sodium-Selenite stock (100 µM, prepared in cell culture grade water, Sigma-Aldrich, Cat# S5261)

add 250 µl of Insulin (10 mg/ml, ready-to-use, Sigma-Aldrich, Cat# I9278)

add 500 µl of G418 stock (100 mg/ml, ready-to-use, InvivoGen, Cat# ant-gn-5)

mix properly

store at 4°C for 4 weeks

temper the medium to room temperature (not 37°C) before use
Protocol preparation of ProxUp medium

Storage conditions

Store the basal medium at 4°C for up to 6 months and the supplements -20°C for up to 6 months.

Store the ready-to-use medium in aliquots at 4°C for up to 1 month..
Temper the medium to room temperature (not 37°C) before use.
Avoid repeated heating to 37°C.
Protocol passaging of RPTEC/TERT1 cells

Protocols

Preparation of cell culture medium ProxUp
Protocol passaging of RPTEC/TERT1

Safety documents (coming soon)

Cell culture media – material safety data sheet

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ProxUp
Ready-to-use – € 109.80,–

Kit components – € 157.50,–

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Related Products

human renal proximal tubular epithelial cells

RPTEC/TERT1

read more

Related Services

send us specific request

Customer Reviews

“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”

 

Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.

“Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform.  We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed.”

Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.

about
our cells

telomerized cells in biotechnology and biomedicine

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Good cell culture practice / GCCP

get ready to standardize your cell culture work

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Related Services

specific request

Customer Reviews

“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”

 

Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.

“Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform.  We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed.”

Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.

Print Friendly, PDF & Email

ProxUp
Cat#: MHT-003 – € 109.80,–

Cat#: MHT-003-B – € 74.80,–

Cat#: MHT-003-S – € 82.70,–

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