Kidney
PodoUp3
PodoUp3 medium support the growth of Evercyte’s human kidney-derived podocytes PODO/TERT256 and urine-derived podocytes PODO/SVTERT152.
General information
Cat#: MHT-033-3 / MHT-033-3-B + MHT-033-3-S
Medium composition
MCDB131 (Pan Biotech, Cat# P04-80057)
1.6 mM GlutaMAX-I (Gibco, Cat# 35050-038, ready-to-use)
9.6 µg/mL BBE (Lonza, Cat# CC-4098, ready-to-use)
8 ng/ml hEGF (Sigma-Aldrich, Cat# E9644)
20 ng/ml Hydrocortisone (Sigma-Aldrich, Cat# H0396)
20 % FBS (PAN Biotech, Cat# P30-3031, ready-to-use)
100 µg/ml G418 (InvivoGen, Cat# ant-gn-5, ready-to-use)
FAQs
Preparation of PodoUp medium
The PodoUp3 medium for cultivation of PODO/TERT256 and PODO/SVTERT152 cells can either be ordered from Evercyte as ready-to-use medium (Cat#MHT-033-3) or as basal medium (MHT-033-3-B) + Supplements (MHT-033-3-S) or can be prepared by mixing the following components:
Storage conditions
Store the basal medium at 4°C for up to 6 months and the supplements -20°C for up to 6 months.
Customer Reviews
“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”
Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.
“Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform. We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed.”
Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.
PODO/TERT256
telomerized podocytes
Customer Reviews
“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”
Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.
“Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform. We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed.”
Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.