PodoUp2021-08-01T20:32:24+00:00

Kidney

PodoUp3

PodoUp3 medium support the growth of Evercyte ́s human kidney-derived podocytes PODO/TERT256 and urine-derived podocytes PODO/SVTERT152.

General information

Cat#: MHT-033-3 / MHT-033-3-B + MHT-033-3-S

Quantity: 500 ml
Format: ready-to-use medium (MHT-033-3) or kit (basal medium / MHT-033-3-B + supplements / MHT-033-3-S)
Storage temperature:
ready-to-use medium / 4°C, basal medium / 4°C, supplements / -20°C
Shelf life:
ready-to-use medium / 1 month, basal medium / 6 months, 4°C, supplements / 6 months
Application:
growth of human podocytes PODO/TERT256 (Evercyte, Cat# CHT-033-0256) and PODO/SVTERT152 (Evercyte, Cat# CLHT-033-0152)

Medium composition

MCDB131 (Pan Biotech, Cat# P04-80057)

1.6 mM GlutaMAX-I (Gibco, Cat# 35050-038, ready-to-use)

9.6 µg/mL BBE (Lonza, Cat# CC-4098, ready-to-use)

8 ng/ml hEGF (Sigma-Aldrich, Cat# E9644)

20 ng/ml Hydrocortisone (Sigma-Aldrich, Cat# H0396)

20 % FBS (Sigma-Aldrich, Cat# F7524, ready-to-use)

100 µg/ml G418 (InvivoGen, Cat# ant-gn-5, ready-to-use)

FAQs

Can the medium be stored for more than 1 month?2021-06-21T09:36:12+00:00

The medium contains temperature sensitive ingredients and therefore it is not recommended to use the medium for a prolonged period of time. Moreover, we recommend our customers to aliquot the medium and store aliquots at 4°C until use. Before use, temper the aliquots at room temperature (not at 37°C).

Preparation of PodoUp medium

The PodoUp3 medium for cultivation of PODO/TERT256 and PODO/SVTERT152 cells can either be ordered from Evercyte as ready-to-use medium (Cat#MHT-033-3) or as basal medium (MHT-033-3-B) + Supplements (MHT-033-3-S) or can be prepared by mixing the following components:

take one bottle (500 ml) of MCDB131 (Pan Biotech, Cat# P04-80057)

add 5 ml GlutaMAX-I (200 mM stock, ready-to-use, Gibco, Cat# 35050-038), mix properly

discard 105 ml from MCDB131 / GlutaMAX-I mixture

add 533.6 µl BBE (9 mg/ml stock, ready-to-use, Lonza, Cat# CC-4098)

add 200 µl hEGF stock (20 µg/ml, prepared in cell culture grade water, Sigma-Aldrich, Cat# E9644)

add 200 µl hydrocortisone stock (50 µg/ml, prepared in cell culture grade water, Sigma-Aldrich, Cat# H0396)
add 100 ml FBS (ready-to-use, Sigma-Aldrich, Cat# F7524)

add 500 µl G418 stock (100 mg/ml, ready-to-use, InvivoGen, Cat# ant-gn-5)

mix properly and store at 4°C for up to 1 month

temper the medium to room temperature (not 37°C) before use

Protocol preparation of PodoUp medium

Storage conditions

Store the basal medium at 4°C for up to 6 months and the supplements -20°C for up to 6 months.

Store the ready-to-use medium in aliquots at 4°C for up to 1 month..
Temper the medium to room temperature (not 37°C) before use.
Avoid repeated heating to 37°C.
Protocol passaging of PODO/TERT256 cells
Protocol passaging of PODO/TERTSV152 cells

Protocols

Preparation of cell culture medium PodoUp
Protocol passaging of PODO/TERT256
Protocol passaging of PODO/SVTERT152

Safety documents (coming soon)

Cell culture media – material safety data sheet

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PodoUp3
Ready-to-use – € 193.50,–

Kit components – € 222.50,–

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Related Products

human telomerized podocytes

PODO/TERT256
PODO/SVTERT152

Related Services

send us specific request

Customer Reviews

“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”

 

Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.

“Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform.  We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed.”

Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.

about
our cells

telomerized cells for the establishment of relevant and standardizable in vitro tests

read more

Good cell culture practice / GCCP

get ready to standardize your cell culture work

read more

Related Products

MyoUp MHT-040

Related Services

specific request

Customer Reviews

“I have had the pleasure of working with Evercyte for the last few years. We continually rely on Evercyte because of the high-quality data that they produce, their diligent responsiveness, and their excellent customer service.”

 

Josh Garlich, Senior Research Scientist, Apellis Pharmaceuticals, Inc.

“Cytonus has been working with Evercyte from many years as they are a trusted partner and have always delivered the highest quality cell lines to advance our platform.  We routinely draw on their expertise to meet cellular engineering challenges and they have not disappointed.”

Remo Moomiaie-Qajar, Cytonus Therapeutics, Inc.

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PodoUp
Cat#: MHT-033-3 – € 193.50,–

Cat#:MHT-033-3-B – € 97.00,–

Cat#:MHT-033-3-S – € 125.50,–

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