Personalized service offer –
EV enrichment and potency

Customer information

Enrichment of extracellular vesicles from cell culture supernatant

If you are sending cell culture supernatants for enrichment / purification of extracellular vesicles, please specify amount of conditioned medium, method for enrichment, purification

Enrichment / purification method requested

tangential flow filtration
ultra-centrifugation
size exclusion

Characterization of EV preparations

Please provide information on degree of characterization that is requested

Particle size and number (Nanoparticle Tracking Analysis)
Presence of EV proteins (western blot / CD81, CD63, Syntenin, no Calnexin)
Presence of EV proteins (MACSplex)
Electron microscopy
Protein content (BCA assay)

Potency / functional assay - general

Please provide information on the number of samples that you would like to be tested.

 

Testing of cells from different donors

Potency / functional assay - specific

Please provide information on the bioassay that you are interested in.

 

 

Neo-angiogenic property

If yes,

Anti-inflammatory activity

If yes,

Anti-fibrotic activity

If yes,

Fibroblast growth promoting activity

If yes,

Fibroblast migration promoting activity / scratch assay

If yes,

Additional

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